TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| OT11 | Pvd+ | 19 |
| OT11pvdS | pvdS::Kan Pvd− | 4 |
| Plasmids | ||
| pMP190 | Broad-host-range promoter probe vector; Cmr | 22 |
| pUC19 | Cloning vector: Apr | 28 |
| pUC::pvdE/F | pvdE-pvdF promoter region (−91 to +34 of pvdF) cloned into pUC19 | 27 |
| pUC::mut1 | pvdE-pvdF region with mutation in pvdE-proximal IS box | This study |
| pUC::mut2 | pvdE-pvdF region with mutation in pvdF-proximal IS box | This study |
| pUC::pvdD | pvdD promoter (−96 to +39) cloned into pUC19 | 27 |
| pUC::pvdDmut | pvdD promoter (−96 to +39) with mutation in IS box | This study |