TABLE 2.
IS boxes in PvdS-dependent promotersa
| Promoter | IS box | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pvdA | −38 | C | T | T | A | A | A | T | T | C | −30 |
| pvdD | −39 | G | C | T | A | A | A | T | C | C | −29 |
| pvdE | −23 | G | C | T | A | A | A | T | A | C | −15 |
| pvdF | −38 | C | G | T | A | A | A | T | T | G | −30 |
| ptxRb | ? | A | G | T | A | A | A | T | A | G | ? |
| pvdY | ? | G | G | T | A | A | A | T | T | C | ? |
| Consensus | (G/C) | (G/C) | T | A | A | A | T | (T/A) | (C/G) | ||
a
The pvdA, -D, -E, and -F genes have been characterized and are required for pyoverdine synthesis (11, 12, 13, 26). The IS boxes of these genes have been mutated and shown to be required for activity (8, 20; this study). Expression of ptxR, which encodes a positive regulator of the pvc genes, and the pvdY gene, which encodes a 17-kDa protein of unknown function, has been shown to be PvdS dependent (24, 25). ?, the transcriptional start site is not known.
b
The transcriptional start site can be estimated on the basis of RNase protection data (24) as being between nucleotide −28 and −20 downstream from the IS box.