TABLE 2.
Primers used to amplify the internal fragments of the given gene of pCLP
| ORFa no. (homolog) | Sequence of:
|
Size of product (bp) | |
|---|---|---|---|
| Forward primer (5′–3′) | Antisense primer (5′–3′)b | ||
| 1 (Rv3128c) | CCTGGGCGCCCTCAAATCCG | GCAGCTCTGATTCTTGGGCC | 424 |
| 2 (pemI) | CCCGGATGGAGGTGGGCG | GCTTCACCGTTGAGCC | 255 |
| 3 (pemK) | ATTGCTCTGACGGAACGCGG | CCCGCCGCGTGTTATAGGTAGC | 152 |
| 2 and 3 (pem operon) | GGACCCCCAGGTGCGACCGC | CCGTTCGGGCAAAGTTCCCG | 152 |
| 4 | CCGGCAAGACCTCGTCAGCC | GCCAATGAGTAGTCCAGCGG | 277 |
| 5 | GGACTACTCGTGTGGCGACAGC | GCTGACGCGGCACAGGAACC | 272 |
| 6 | GGTCGCCCGTGAATACCGG | GCATCTTGCGCCCAATCGCC | 293 |
| 7 | CGAGCGACGGTGATCCGTACCG | GCGTCTATTGATGCCGAACACC | 316 |
| 8 (Rv2813) | CGTCATCTATCTGCCTGACC | CGCGGCCGGCCAGCTTCAGG | 413 |
| 9 (Rv2812) | GGCAGACCCCGCTGGCCCGC | CGGCGCGCCGGCCAGCCGC | 200 |
| 10 | CGCAGCCCGCGCGGGACAGC | GGCGTCGAGTGCGCTCCGCC | 223 |
| 11 (parA) | GCCGTTGCCGGTTTCCCTCG | CGGTTGATCGCCCACACCGC | 253 |
| 12 | CCGCGCCGGCCTAGCGCCG | CGGCGGTGTCGGCGTGCTGG | 102 |
| 13 | GCGCGCAGGCACGCGGACG | CCCCCAGGTGGGGGCGACCC | 288 |
| 14 (rep) | GCCGGGCGTGGATCGAACGG | ATCCGGCCGCCCCAAGAACC | 301 |
| 15 | GCCGGCGTCGGTGGTGGCG | GCTCGACCCACCGGCAGCCGG | 264 |
| 16 (Rv0921) | GGGGAATCCGGCCACACGG | CGGCGCGCCGGCCAGCCGC | 310 |
| 17 (Rv0922) | GGCGCCGAAGTCGTTGACCG | GGGCGGCATCAGCCAGGGC | 316 |
| 18 | CGGCCCCGCCGACGCACCGC | GCCCGCGACGAAGCGTCGGG | 277 |
| 19 | CCGCCAGCGGCGTCATTGG | GGTCCTGCCGACCCGCGGCG | 268 |
a
See Fig. 1 for location of ORFs on pCLP.
b
These primers were also used to prime cDNA.