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. 2022 Sep 12;3:991725. doi: 10.3389/fpain.2022.991725

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Copyright © 2022 Horan, McIlvried, Atherton, Yuan, Dolan and Scheff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Oral cancer-induced changes in pain-related protein expression. Each TG tissue sections from each retrogradely labeled mouse was stained with antibodies specific for either TRPV1, CGRP or ATF3. Representative immunostaining of all three markers in retrogradely labeled TG tissue from (A) sham and MOC1 mice at PID40 as well as (B) sham and MOC2 mice at PID14. (C,D) The percentage of DiI-labeled neurons expressing each marker was quantified from 2–4 animals per treatment group. Data are presented as mean +/- SEM of percentage of neurons expressing each marker. Two-way ANOVA, Treatment effect: ^*p < 0.05, ^**p < 0.01, sex effect: #p < 0.05.