Figure 5.

Tumor-associated immune infiltrate during tumor progression. Immune infiltrate in tongue tissue was measured in male and female sham, MOC1 and MOC2 tumor-bearing mice during tumorigenesis. (A) Analytical cytometric gating strategy used to characterize immune subpopulations. Following viability gating to isolate live cells (not shown), CD45+ cells were gated into either CD3+/- lymphocytes or CD11b+ myeloid cells. CD3+ cells were further gated into CD4 T helper or CD8 cytotoxic T cells. CD3- cells were further gated into CD19+ B cells or NK1.1+ NK cells. The CD11b+ leukocytes were gated into MHCII+CD11c+ dendritic cells, Ly6G-F4/80+ macrophages, or CD11b+Ly6g+ neutrophils. (B) Representative scatter plots showing CD45+ immune cells in tongue tissue from sham and MOC1 tumors at PID20 as well as in sham and MOC2 tumors at PID6. Histograms demonstrate the increase in CD45+ counts in tumor tongue tissue compared to sham. CD45+ immune infiltrate was quantified by analytical cytometry at three different time points in the MOC1 (C) and MOC2 (D) models. N = 3 mice/sex/timepoint. Within sex comparisons were done to assess the effect of treatment (**p < 0.01) and time (#p < 0.05) by Two-way ANOVA.