Table 6.
Muscle metabolites of beef feedlot steers fed different concentrations of Zn and transported for 18 h
| Pretransit3 | ||||||||
|---|---|---|---|---|---|---|---|---|
| IND1 vs. CON | SUPZN vs. CON | IND vs. SUPZN | ||||||
| Name | FC2 | P-value2 | Name | FC | P-value | Name | FC | P-value |
| 5-Oxoproline | 0.64 | 0.03 | Urea | 1.41 | 0.01 | Urea | 0.73 | 0.03 |
| 3-Hydroxybutyric acid | 1.63 | 0.09 | Lactic Acid | 1.29 | 0.03 | L-Alanine | 0.69 | 0.03 |
| Serine | 0.75 | 0.10 | L-Valine | 1.28 | 0.03 | L-Proline | 0.75 | 0.03 |
| 3-Hydroxybutyric acid | 1.89 | 0.03 | L-Valine | 0.77 | 0.05 | |||
| 5-Oxoproline | 0.74 | 0.05 | ||||||
| Lactic Acid | 0.79 | 0.06 | ||||||
| Allothreonine | 0.72 | 0.06 | ||||||
| Malic acid | 0.71 | 0.09 | ||||||
| Butanedioic acid | 0.72 | 0.10 | ||||||
| Posttransit4 | ||||||||
|---|---|---|---|---|---|---|---|---|
| IND vs. CON | SUPZN vs. CON | IND vs. SUPZN | ||||||
| Name | FC | P-value | Name | FC | P-value | Name | FC | P-value |
| L-Phenylalanine | 0.67 | 0.06 | D-Glucose | 0.52 | 0.01 | |||
| D-Glucose | 0.56 | 0.10 | L-Norleucine | 1.32 | 0.04 | |||
| L-Phenylalanine | 0.61 | 0.04 | ||||||
| L-Valine | 1.26 | 0.08 | ||||||
| Posttransit v Pretransit within a single Zn treatment | ||||||||
|---|---|---|---|---|---|---|---|---|
| CON | IND | SUPZN | ||||||
| Name | FC | P-value | Name | FC | P-value | Name | FC | P-value |
| p-Toluic acid | 2.38 | 0.01 | Allothreonine | 2.51 | 0.01 | p-Toluic acid | 2.49 | 0.01 |
| D-Glucose | 3.83 | 0.01 | p-Toluic acid | 2.95 | 0.01 | Allothreonine | 2.20 | 0.01 |
| Galactopyranose | 3.03 | 0.01 | Serine | 1.91 | 0.01 | Serine | 1.89 | 0.01 |
| Allothreonine | 2.03 | 0.01 | Butanedioic acid | 1.64 | 0.03 | Malic acid | 0.60 | 0.02 |
| Serine | 1.63 | 0.02 | L-Alanine | 1.57 | 0.06 | Myristic acid | 1.72 | 0.02 |
| L-Phenylalanine | 1.52 | 0.02 | 3-Hydroxybutyric acid | 0.70 | 0.07 | Octanoic acid | 1.67 | 0.07 |
| Hydroxyproline | 1.61 | 0.04 | Lactic acid | 1.47 | 0.07 | Octadecanoic acid | 1.54 | 0.08 |
| L-Glutamine | 3.52 | 0.04 | L-Glutamic acid | 0.71 | 0.08 | |||
| Lactic acid | 1.65 | 0.04 | ||||||
| L-Glutamic acid | 0.69 | 0.06 | ||||||
| L-Alanine | 1.53 | 0.07 | ||||||
| L-Norleucine | 0.75 | 0.09 | ||||||
CON, no supplemental Zn (n = 12 steers); IND, 70-mg supplemental Zn/kg DM (n = 12 steers); SUPZN, 120-mg supplemental Zn/kg DM (n = 11 steers). All supplemental Zn provided through Plexomin Zn (Phytobiotics, Cary, NC).
Identified muscle metabolites were analyzed in MetaboAnalyst 5.0 (Chong et al., 2018; Xia Lab, McGill, CA) using a student’s t-test and differential abundance classification (FC ≥ 1.25; P ≤ 0.1). Differences were reported as fold changes (FC; FC = first contrast parameter AUC/second contrast parameter AUC).
Pretransit samples were collected postfeed access on the day before the 18-h transit event (day –1).
Posttransit samplers were collected the day steers arrived back to the farm after steers had been weighed, had blood drawn, and had access to feed (d 1)