Figure 4.

H3 and H4 augment LPS- and TNF-α-induced inflammatory response in human pulmonary endothelium. HPAECs were stimulated with LPS (25 ng/mL) or TNFα (0.1 ng/mL) alone or combined with 20 µg/mL of indicated histone subunit. A: ICAM-1 and VCAM-1 protein expression was evaluated by Western blotting. B: the levels of secreted sICAM-1, IL-6, and IL-8 were determined by ELISA. Data represent means ± SD, n = 5, *P < 0.05 vs. LPS or TNFα alone. HPAEC, human pulmonary artery endothelial cell; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α.