Table 4.

Accelerating Rota-Rod

	Group				P Values
	SC-C	SC-DMB	WD-C	WD-DMB	Group × Time	Diet × Treatment	Diet	Treatment
Longest running time, s
Baseline	180 ± 14	184 ± 12	176 ± 19	163 ± 14		0.557	0.39	0.74
8 Wk	250 ± 20‡	265 ± 19‡	164 ± 15*	191 ± 14	<0.01	0.71	<0.0001	0.24
Maximal running speed, revs/min
Baseline	25 ± 2	26 ± 2	25 ± 2	23 ± 2		0.56	0.39	0.74
8 Wk	34 ± 2‡	36 ± 2‡	23 ± 2*	27 ± 2	<0.01	0.71	<0.0001	0.24

Data presented are the longest time or maximal speed attained across 3 accelerating rota-rod trials, separated by ∼1 h each. Groups are: standard chow-control water (SC-C), standard chow-DMB water (SC-DMB), Western diet-control water (WD-C), and Western diet-DMB water (WD-DMB). Data are means ± SE. n = 10–13 mice/group (1 statistical outlier removed from WD-C group at 8 wk). Statistics are two-way mixed (group × time) ANOVA and, within each time point, two-way diet × treatment ANOVA. Symbols are pairwise comparisons (Tukey’s post hoc test) from the two-way group × time ANOVA as follows: *P < 0.05 vs. SC-C within time point. ‡P < 0.05 vs. baseline within group.