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. 2022 Sep 23;6(3):NS20220013. doi: 10.1042/NS20220013

Figure 3. Basic image editing workflow.

Figure 3

(A) Example of a stacked AT image, consisting of 20 serial 70 nm sections. Synaptophysin (presynapse) in magenta and PSD95 (postsynapse) in cyan. (B) Example of the same image after segmentation, which removes background puncta present in only one 70 nm section and binarizes the retained synaptic puncta which are present in at least two consecutive sections. (C) Example of 3D-rendered synapses from (B). See the close and direct apposition of the re and post-synaptic markers.