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Optimization of RAA-LFD reaction conditions. The optimal primer concentration was 0.625μM, the reaction temperature was 40°C and the reaction time was 30 min. (A) screening of primer and probe concentration; (B) screening of reaction temperature; (C) screening of reaction time. +: positive group (plasmid pGEM-T-TNFR, 105copies/μL). −: negative control (water). C, control line; T, test line.
