Table 1.
The sequences of primers and probes designed in this study.
| Gene name | Prime name | Sequences (5’-3’) | Product size(bp) | Function |
|---|---|---|---|---|
| TNFR-ORF146R | RAA-F1 | TGTTCGACTCATACCCCTACCCACCAGACTA | 139 | RAA |
| RAA-R1 | ATCGAGAGCAACGTCGGTTTCCAACCATTCA | |||
| RAA-F2 | CTGTTCGACTCATACCCCTACCCACCAGACTACC | 148 | ||
| RAA-R2 | ACTCGCGGATCGAGAGCAACGTCGGTTTCCAACC | |||
| RAA-F3 | CTGTTCGACTCATACCCCTACCCACCAGACTAC | 138 | ||
| RAA-R3 | CGAGAGCAACGTCGGTTTCCAACCATTCACTG | |||
| RAA-F4 | CTAATCGCTGTCCTCTTGACCCTATCGGGCCTGGA | 98 | ||
| RAA-R4 | GTTGGCTTGGTGATTGCACGTCTGGTGGGTAGTCT | |||
| RAA-F5 | TGCTTCTAATCGCTGTCCTCTTGACCCTATCG | 108 | ||
| RAA-R5 | TGATTGTTGGCTTGGTGATTGCACGTCTGG | |||
| RAA-LF | CTGTTCGACTCATACCCCTACCCACCAGACTACC | RAA-LFD | ||
| RAA-LR | Biotin-ACTCGCGGATCGAGAGCAACGTCGGTTTCCAACC | |||
| RAA-Probe | FAM-CTACCCACCAGACTACCCACCAGACGTGCAA(THF)TCACCAAGCCAACAATC-Spacer | |||
| PCR-F | ACCCCTACCCACCAGACTAC | 789 | PCR | |
| PCR-R | TCGAGGTTCGTTTTGGCGTA |