Table 3. Results of tTMB and bTMB using different approach of TMB calculation and our gene panel LMB_TMB1.
| Histology | tTMB alla) | bTMB allb) | tTMB non-synomyous allc) | bTMB non-synomyous alld) | tTMB nonHLAe) | bTMB nonHLAf) | tTMB non-synomyous nonHLAg) | bTMB non-synomyous nonHLAh) | |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Aca | 52 | 35 | 38 | 28 | 44 | 8 | 31 | 5 |
| 2 | Aca | 44 | 56 | 35 | 41 | 33 | 41 | 24 | 31 |
| 3 | Aca | 41 | 43 | 27 | 28 | 21 | 23 | 14 | 14 |
| 4 | Aca | (17) | (20) | 14 | 14 | 14 | 5 | 11 | 5 |
| 5 | Aca | 53 | 30 | 41 | 22 | 41 | 11 | 33 | 8 |
| 6 | Aca | (6) | (17) | 4 | 11 | (5) | (4) | 3 | 3 |
| 7 | Aca | 27 | 42 | 20 | 28 | 21 | 11 | 16 | 8 |
| 8 | Aca | 30 | 46 | 25 | 37 | 10x | 6x | 8 | 5 |
| 9 | Aca | (16) | (10) | 15 | 7 | (11) | (4) | 11 | 4 |
| 10 | Aca | 11x | 33x | 5 | 21 | 6x | 9x | 5 | 6 |
| 11 | Aca | 77 | 21 | 58 | 9 | 76 | 8 | 58 | 4 |
| 12 | SCC | 27 | 22 | 19 | 20 | 14 | 6 | 10 | 6 |
| 13 | SCC | 35 | 30 | 25 | 19 | 23 | 6 | 19 | 3 |
| 14 | SCC | 60 | 69 | 48 | 51 | 35 | 19 | 28 | 14 |
| 15 | SCC | (21) | (10) | 19 | 5 | (9) | (4) | 8 | 3 |
| 16 | SCC | 53 | 53 | 36 | 37 | 23 | 6 | 16 | 4 |
| 17 | SCC | 23 | 35 | 20 | 25 | 15 | 5 | 11 | 4 |
| 18 | SCC | 19x | 40x | 16 | 31 | 8x | 11x | 6 | 9 |
| 19 | SCC | 26 | 21 | 16 | 12 | 15 | 9 | 9 | 5 |
| 20 | SCC | 54 | 57 | 44 | 42 | 24 | 5 | 21 | 5 |
Aca = adenocarcinoma; SCC = spinocellular carcinoma.
Bold = Matching TMB positivity (= high TMB) in tissue and serum using all mutation calculation in/excluding HLA genes (tissue ≥22/12 mut/Mb and serum ≥21/5 mut/Mb).
() = Matching TMB negativity (= low TMB) in tissue and serum using all mutation calculation in/excluding HLA genes (tissue <22/12 mut/Mb and serum <21/5 mut/Mb).
x Mismatch in TMB positivity/negativity between tTMB and bTMB in the same TMB calculation approach with defined cut-offs.
a) tTMB all = all tissue somatic mutations in all genes.
b) bTMB all = all liquid biopsy somatic mutations in all genes.
c) tTMB non-synonymous all = all tissue non-synonymous somatic mutations in all genes.
d) bTMB non-synonymous all = all liquid biopsy non-synonymous somatic mutations in all genes.
e) tTMB nonHLA = all tissue somatic mutations in all genes except HLA genes.
f) bTMB nonHLA = all liquid somatic mutations in all genes except HLA genes.
g) tTMB non-synonymous nonHLA = all tissue non-synonymous somatic mutations in all genes except HLA genes.
h) bTMB non-synonymous nonHLA = all liquid biopsy non-synonymous somatic mutations in all genes except HLA genes.