Table 4. Comparison of currently used gene panels for tTMB calculation [30, 31] with our panel (LMB_TMB1).

Gene panel	Number of genes	Size region covered* (Mb)	TMB cut-off	Gene variants included in TMB calculation	FDA approved
F1CDx	324	0.8 Mb	10 mut/Mb	Somatic non-synonymous and synonymous mutations	Yes
MSK-IMPACT	468	1.14 Mb		Somatic non-synonymous mutations	Yes
Illumina TSO500	523	1.33 Mb	10 mut/Mb	Somatic non-synonymous and synonymous mutations	No
Oncomine TML	409	1.22 Mb		Somatic non-synonymous mutations	No
QIAseq TMB	486	1.33 Mb		Somatic non-synonymous mutations	No
LMB_TMB1	313	0.81/0.8 Mb**	22/12 mut/Mb**	Somatic non-synonymous and synonymous mutations***	No

* Coding region used to estimate TMB regardless of the size of the region assessed by the panel.

** The approach for TMB calculation including HLA genes / the approach for TMB calculation excluding HLA genes.

*** Non-synonymous mutations include SNPs and short insertions and deletions (indels).