FIG. 5.
Mutations in groE and dnaK do not compromise the viability of SRP-deficient cells. (A) Strains HDB92 (Ptrc-ffh ffh::kan), HDB93 (Ptrc-ffh ffh::kan groEL44), and HDB94 (Ptrc-ffh ffh::kan groES619) were streaked on LB agar containing either 10 μM IPTG or no IPTG. Plates were incubated at 35 and 42°C for 20 and 16 h, respectively. Cells were diluted from overnight cultures and incubated at 35 and 42°C on LB agar plates containing either 10 μM IPTG or no IPTG to determine relative plating efficiencies as described in Materials and Methods. (B) Strains HDB95 (Plac-ffh ffh::kan) and HDB96 (Plac-ffh ffh::kan ΔdnaK52 sidB1) were streaked on LB agar containing either 200 μM IPTG or 50 μM IPTG. Cells were diluted from overnight cultures and incubated at 30°C on LB agar plates containing either 200 μM IPTG or 50 μM IPTG to determine relative plating efficiencies. In both panels A and B, plating efficiency was plotted on a logarithmic (log10) scale.