Skip to main content
. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Immunol. 2022 Aug 5;209(5):864–873. doi: 10.4049/jimmunol.2200145

Figure 5. The viral accessory proteins Nef and Vpu do not impact the presentation of the Gag293 epitope by DCs and activated TCD4+.

Figure 5.

DCs and activated TCD4+ were cultured and infected with WT HIV-1 and Nef-/Vpu- HIV-1 as described in Materials and Methods. (A) DCs and (B) TCD4+ were cocultured with HIV-1-specific TCD4+ for 8 hours and IL-2, TNFα, and IFNγ expression was assessed by flow cytometry. Fold induction of each cytokine is shown, using DMSO as a baseline. Each dot represents an independent experiment. Bars represent mean ± SD. One way ANOVA, ****p<0.001.