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. 2022 Sep 2;11:e65987. doi: 10.7554/eLife.65987

Figure 1. PIEZO1 is functionally expressed in mouse and human keratinocytes.

(A) RNAscope of hindpaw glabrous skin isolated from wildtype and PIEZO1cKO mice targeting PIEZO1 mRNA (blue: DAPI, red: PIEZO1). (B) PIEZO1 gene expression was measured in keratinocytes isolated from wildtype (wt; n=3) and PIEZO1cKO (n=3) mice using quantitative real-time PCR. Expression levels were normalized to HPRT. Piezo1 expression was undetected in PIEZO1cKO samples. (C) Average calcium flux in wildtype (wt) and PIEZO1cKO keratinocytes in response to 1000 nM Yoda1; trace outline is SEM. (D) Percentage of wildtype and PIEZO1cKO keratinocytes that respond to extracellular Yoda1; cells from n=3 mice per genotype; bars are group averages; Chi square. (E) Calcium flux in human keratinocytes in response to 1000 nM Yoda1; trace outline is SEM. (F) Percentage of human keratinocytes that respond to extracellular Yoda1; cells from the skin of n=3 human donors; bars are group averages. All data are mean ± SEM unless otherwise stated. Post-hoc comparisons for all panels: **p<0.01, ****p<0.0001.

Figure 1—source data 1. Data for panals Figure 1B-F.

Figure 1.

Figure 1—figure supplement 1. The epidermis of PIEZO1cKO animals has normal morphological features.

Figure 1—figure supplement 1.

(A) H&E stained skin from the hindpaw of wildtype (wt) and PIEZO1cKO mice. (B) Quantification of individual epidermal layer thickness; 2-way ANOVA, n.s. All data are mean ± SEM.
Figure 1—figure supplement 1—source data 1. Individual values for epidermal thickness.