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. 2022 Sep 2;11:e65987. doi: 10.7554/eLife.65987

Figure 2. PIEZO1 deletion decreases keratinocyte mechanical sensitivity.

Figure 2.

(A) Examples of whole-cell recording of mechanically activated (MA) current (Vh = –40 mV) evoked in keratinocytes by a stepwise increase in membrane indentation depth. (B) The mechanical threshold to evoke MA current by gradual increasing of indentation depth is significantly increased in keratinocytes of PIEZO1cKO mice compared to wild-type controls; Mann-Whitney U-test. (C) PIEZO1 deletion significantly decreased number of keratinocytes that responded to membrane indentation with MA current (wildtype: n=23 cells; PIEZO1cKO: n=37 cells); Chi square test. (D) Representative traces of rapidly adapting (RA), intermediately adapting (IA), and slowly adapting (SA) MA currents induced in keratinocytes in response to membrane indentation. (E) Proportion of keratinocytes that responded to membrane indentation with RA, IA, and SA currents is not affected by PIEZO1 deletion (n=18 recordings of MA current per each group); Chi square and Fisher’s exact post hoc test, n.s. (F) Maximal amplitude of MA currents in wild-type and PIEZO1cKO keratinocytes; Mann-Whitney U-test, n.s. For whole-cell patch clamp experiments, cells were harvested from n=5 mice per group. All data are mean ± SEM. *p<0.05.

Figure 2—source data 1. Data for mechanical threshold, percent responders, current profile and max amplitude of wt and PIEZO1cKO keratinocytes.