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. 2022 Sep 2;11:e65987. doi: 10.7554/eLife.65987

Figure 4. Yoda1 induces paw attending behaviors and C fiber mechanical hypersensitivity.

Figure 4.

(A) Yoda1-induced attending behaviors in wildtype mice; Kruskal Wallis test. (B) 1 mM Yoda1 induced attending behaviors in wildtype and PIEZO1cKO mice; 2-way ANOVA. (C) Example traces from wildtype preparations exposed to 1 mM Yoda1 or vehicle during mechanical testing. (D) Mean mechanical firing frequency of C fibers from wildtype animals exposed to 1 mM Yoda1 or vehicle. (E) Example traces of PIEZO1cKO preparations exposed to 1 mM Yoda1 or vehicle during mechanical testing. (F) Mean mechanical firing frequency of C fibers from PIEZO1cKO animals exposed to 1 mM Yoda1 or vehicle. Fibers from n=12–14 mice. For all teased fiber recordings, the mechanical stimulus was applied to the skin for 10 s; 2-way ANOVA. (G) Von Frey mechanical thresholds of wildtype and PIEZO1cKO mice tested 30 min after an injection of 1 mM Yoda1 or vehicle; 2-way ANOVA . All data are mean ± SEM unless otherwise stated. Post-hoc comparisons for all panels: **p<0.01, ***p<0.001, ****p<0.0001.

Figure 4—source data 1. Data for time attending to hindpaws, Yoda1 induced firing frequency, and Yoda1 induced mechanical hypersensitivity.