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ACT production by S. lividans (TK21) in the presence of glycerol. Portions (10 μl) of diluted spore stocks of S. lividans TK21, SL41, SL94, and SL94-1 were spotted onto minimal-agar plates containing 0.5% glucose (left), 0.25% glycerol (middle), or both (right) as a carbon source. After a week of incubation at 30°C, each plate was placed upside down for ammonia fuming as well as for photography.
