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Actinorhodin production by S. lividans (TK21, SL94, and SL41) in liquid culture. Each S. lividans was inoculated into a 250-ml baffled flask containing 25 ml of minimal medium that included either glucose or glycerol as a sold carbon source, followed by incubation at 30°C for 3 days with shaking. Samples (0.5 ml) were taken at various time points and measured.
