Appendix 1—table 2. Salient clinical characteristics and single-cell image segmentation details from imaging mass cytometry experiments comparing tissue-level neutrophil-to-lymphocyte ratio (NLR) and stromal α-SMA pixel intensity in pre-chemotherapy tissue sections from localized pancreatic ductal adenocarcinoma (PDAC) patients who demonstrated either partial/complete or poor/absent pathologic response to neoadjuvant chemotherapy.
| Pt # | Primary tumor | NAC regimen | Duration of NAC (mo) | Neoadjuvant radiation | Pathologic response | # total single cells in IMC slide | #CD11b+CD15+ neutrophils | #CD3+CD8+ T cells | NLR (norm. to 5000 cells) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Borderline Resectable | FFX | 4 | No | Partial (CAP 2) |
4326 | 325 | 29 | 11.2 |
| 2 | Borderline Resectable | FFX | 4.5 | No | Partial (CAP 2) |
3256 | 256 | 32 | 7.9 |
| 3 | Locally Advanced* | GNP | 6 | No | Near-complete (CAP 1) | 9421 | 472 | 142 | 3.3 |
| 4 | Borderline Resectable | GNP | 5 | No | Poor/Absent (CAP 3) |
8621 | 951 | 61 | 15.6 |
| 5 | Locally Advanced* | FFX | 6 | No | Poor/Absent (CAP 3) |
6011 | 1105 | 59 | 18.7 |
| 6 | Resectable | FFX +GNP | 6 | No | Poor/Absent (CAP 3) |
4712 | 523 | 40 | 13.1 |
*
Representative tissue and image segmentation maps depicted in Figure 4.