Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) | Pancreatic Tumor Cells from KrasLSL-12D/+;Trp53R172H/+;Pdx1Cre (KPC) mouse | Ben Stanger/UPenn | KPC6694c2 | |
| Cell line (Mus musculus) | Tumor associated fibroblasts from KPC mouse | Nagathihalli et al., 2016 | KPC CAFs | |
| Other | Ptf1aCre/+;KrasLSL-G12D/+;Tgfbr2flox/flox | Datta et al., 2022 | PKT | Genetically engineered mouse |
| Antibody | Anti-Ly6G (Rat monoclonal) reactive to mouse | BioXcell | Clone 1A8 Catalog# BE0075-1 |
25 μg/dose |
| Antibody | Anti-IL-1β neutralizing antibody (E. coli, polyclonal) | R&D Systems | Catalog# AF-401-NA | 1:80 |
| Antibody | Cxcl1 (Rabbit, monoclonal) Reactive to human and mouse | Abcam | Catalog# ab86436 | 1:500 |
| Antibody | Podoplanin (Mouse, monoclonal) Reactive to human | Cell Signalling | Catalog# 26981 | 1:200 |
| Antibody | Podoplanin (Syrian hamster, monoclonal) Reactive to mouse | Abcam | Catalog# ab92319 | 1:200 |
| Antibody | CD3 (170Er, Human, monoclonal) 3170019D | Fluidigm | 3170019D | 1:1000 |
| Antibody | CD11B (149Sm, Human, monoclonal) | Fluidigm | 3149028D | 1:1000 |
| Antibody | α-SMA (141Pr, Human, monoclonal) | Fluidigm | 314017D | 1:1000 |
| Antibody | Pan-Cytokeratin (148Nd, Human, monoclonal) | Fluidigm | 3148022D | 1:1000 |
| Antibody | CD15 (164Dy, Human, monoclonal) | Fluidigm | 3164001B | 1:1000 |
| Antibody | CD8 (146Nd, Human, monoclonal) | Fluidigm | 3146001B | 1:1000 |
| Chemical compound, drug | Anakinra | SOBI Pharmaceuticals |
α-IL-1R1 inhibitor | |
| Sequence-based reagent | Cxcl1 Primer - Mouse | Qiagen | Gene ID - QT00115647 | |
| Sequence-based reagent | Il6 Primer - Mouse | Qiagen | Gene ID - QT00098875 | |
| Commercial assay or kit | Cytokine array - Mouse | R&D Systems | ARY006 |