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. 2022 Aug 24;6(10):2950–2963. doi: 10.1002/hep4.2055

FIGURE 8.

FIGURE 8

The reduction of DNAJB1‐PKAc by inhibition of β‐catenin identified an auto‐regulatory loop that supports high levels of the fusion oncoprotein in patients with FLC. (A) Diagram showing fusion points for DNAJB1‐PKAc isoforms detected in FLC organoids. (B,C) Reduction of transcripts of DNAJB1‐PKAc isoforms in FLC organoids, treated with PRI‐724 (5 μm, n = 4/group; *adjusted p < 0.05). (D) Western blotting of proteins from FLC organoids with antibodies to the N‐Terminal part of DNAJB1 and the C‐terminal part of PKAc. (E) Hypothesis for auto‐regulatory loop. Bottom image shows an additional part of the loop, in which DNAJB1‐PKAc‐β‐catenin‐TCF4 increases levels of TCF4. A sequence of the CEGR/ALCD in the TCF4 gene is shown.