FIGURE 3.

Germline loss of hepatocyte RARα induces hepatosteatosis in high‐fat diet–fed mice. Eight‐week‐old male Rarα ^fl/fl and gL‐Rarα ^−/− mice were fed a high‐fat diet (HFD) (n = 7) or a chow diet (n = 8) for 20 weeks. Plasma AST (A) and ALT (B) levels, and hepatic TG (C) and NEFA (D) levels were determined. (E,F) Representative liver images of Oil Red O staining (E) and H&E staining (F) (scale bars = 20 μm). (G) Hepatic mRNA levels of HFD‐fed mice were measured. All values are expressed as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001. Cpt1, carnitine palmitoyltransferase 1; FA, fatty acid; FAO, fatty acid oxidation; Fabp4, fatty acid binding protein 4; Il1β, interleukin 1β; Tgfβ, tumor growth factorβ.