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. 2022 Jul 19;6(10):2665–2675. doi: 10.1002/hep4.2049

FIGURE 6.

FIGURE 6

AtRA reduces hepatosteatosis by inhibiting hepatocyte fatty acid uptake and lipid droplet formation. Rara fl/fl mice were intravenously injected with AAV8‐TBG‐null or AAV8‐TBG‐iCRE and then gavaged with vehicle or AtRA (15 mg/kg) for 16 weeks (n = 8 per group). These mice were also fed a HFCF diet. Hepatic mRNA levels of genes involved in lipid droplet formation (A), fatty acid uptake (B), and lipogenesis (C) were determined. (D) Hepatic newly synthesized TG levels were analyzed. (E) Fatty acid uptake was determined in primary hepatocytes treated with either vehicle or AtRA (4 μm) (n = 10 per group). (F) FAO was performed in isolated primary hepatocytes treated with either vehicle or AtRA (2 μm), with each well seeded with the same amounts of cells. All values are expressed as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001. RFU, relative fluorescence unit.