Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) basic steps. Complementary DNA (cDNA) is first synthesized by preparing a master mix containing an RNA template and reverse transcriptase enzyme. Then, another master mix that includes gene-specific primers and the enzyme DNA polymerase is added to initiate the PCR reaction, resulting in millions of DNA targeted sequence copies. Real-time fluorescence detection shows the amplification curve for positive samples.
