Figure 5.

Downregulation of PTBP1 in the hippocampus fails to attenuate cognitive and synaptic deficits as well as tau pathology in PS19 mice. A, Quantification of discrimination indexes in the NOR test of PS19 and WT control mice 2.5 months after the shPtbp1 or the scramble control AAV transduction; n = 9–14 animals, one-way ANOVA with Tukey's multiple comparisons. B, Analysis of the freezing behavior of the experimental mouse during the CFC test; n = 11–16 animals, one-way ANOVA with Tukey's multiple comparisons. C, D, Confocal analysis of SYP⁺PSD95⁺ synaptic puncta. Representative images (C), quantification (D); n = 4–5 animals, one-way ANOVA with Tukey's multiple comparisons. E, F, Confocal analysis of AT8 (phospho-tau antibody) stained tau pathology. Representative images (E), quantification (F); n = 4 animals per group, unpaired t test. All quantified data are represented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, Not significant.