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TLC of acyl-HSLs. Ethyl acetate extracts were chromatographed on C18 reversed-phase TLC plates developed with methanol-water (60:40, vol/vol). The spots were visualized using the A. tumefaciens reporter strain. Lane 1, K56-2; lane 2, K56-I2; lane 3, K56-R2; lane 4, Pc715j; lane 5, Pc715j-R1; lane 6, synthetic OHL and HHL standards.
