Skip to main content
. 2022 Sep 20;3(9):100659. doi: 10.1016/j.xcrm.2022.100659

Figure 1.

Figure 1

HNSCC resistant cells are more aggressive and overexpress AXL and c-MET

(A) Naive (33), cisplatin-resistant CAL33 (33R), irradiation-resistant CAL33 (33xR), and double-resistant (33RR) CAL33 cells were incubated with cisplatin for 48 h. Cell viability was evaluated with XTT assays. N = 4.

(B) Naive (27), cisplatin-resistant CAL27 (27R), and double-cisplatin- and irradiation-resistant (27RR) CAL27 cells were incubated with cisplatin for 48 h. Cell viability was evaluated by XTT assays. N = 4.

(C and D) 33, 33R, 33xR, 33RR, 27, 27R, and 27RR cells were irradiated with 4 Gy, and the clonogenic potential was evaluated after 10 days (see Figure S1B). N = 3.

(E and F) Serum-stimulated cell migration after 24 h was analyzed using Boyden chamber assays for 33, 33R, 33xR, 33RR, 27, 27R, and 27RR cells (see Figure S1C). N = 3.

(G and H) 33, 33R, 33xR, 33RR, 27, 27R, and 27RR cell invasion after 3 days was evaluated using spheroid assays (see Figure S1D). N = 3.

(I and J) AXL and MET mRNA levels in 33, 33R, 33xR, 33RR, 27, 27R, and 27RR cells were determined by qPCR. Control conditions were considered as the reference value (1). N = 3.

(K and L) Phospho-AXL (p-AXL), AXL, p-MET, and MET were evaluated by immunoblotting of 33, 33R, 33xR, 33RR, 27, 27R, and 27RR cells. HSP60 served as a loading control. N = 3. Statistics were performed using the ANOVA test: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.