Figure 6.

Cabozantinib inhibits appearance of HNSCC metastases and decreases the size of pre-existing HNSCC metastases in zebrafish

(A–D) Zebrafish embryos (N = 30) were injected with 33RR cells (labeled with red DiD) into the perivitelline space and treated with cabozantinib for 72 h (1 μM). Zebrafish embryos were monitored for tumor metastases using a fluorescent microscope. Representative images are shown, and the area of metastases was quantified.

(A) Representative images of local and distant metastases. Arrow indicates injection space at t0.

(B) Percentage of fish with distant metastases after 24 h.

(C) Evaluation of invasion of local metastases after 72 h.

(D) Quantification of the area of distant metastases during 72 h.

(E and F) Zebrafish embryos (n = 20) were injected with 33RR cells (labeled with red DiD) into the perivitelline space. Twenty-four h later, only zebrafish with metastases were chosen and treated for 48 h with cabozantinib (1 μM). Zebrafish embryos were monitored to visualize tumor spreading using a fluorescent microscope.

(E) Representative images are shown.

(F) Comparison of the area of distant metastases between the cabozantinib- and control-treated groups. Representative images are shown. Statistics were performed using the ANOVA test: ∗p < 0,05, ∗∗∗p < 0.001.