REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-LR11 (clone 48/LR11)	BD Biosciences	Cat#612633; RRID: AB_399670
Mouse monoclonal anti-Tau (clone 5E2)	Millipore	Cat#05-348; RRID: AB_309687
Mouse monoclonal anti-phospho-Tau (Ser202, Thr205, clone AT8)	Thermo Fischer Scientific	Cat#MN1020; RRID: AB_223647
Rabbit monoclonal anti-Rab5 [EPR5438]	Abcam	Cat#ab109534; RRID: AB_10865740
Mouse monoclonal anti-β-Actin (clone AC-15)	Sigma-Aldrich	Cat#A5441; RRID: AB_476744
Rabbit polyclonal anti-SORLA (pAb_5387), in-house	DAKO	https://pabmabs.com/?p=2318 and https://doi.org/10.1074/jbc.M100857200
Rabbit polyclonal anti-APP1227, in-house	Olav Andersen Lab, Aarhus University, Aarhus, Denmark	https://www.pnas.org/doi/full/10.1073/pnas.0503689102
Rabbit polyclonal anti-beta Amyloid 1-42 (Aβ42)	Abcam	Cat#ab10148; RRID: AB_296881
Rabbit polyclonal anti-VPS26a	Abcam	Cat #ab23892; RRID: AB_2215043
Rabbit polyclonal anti-VPS26b	Abcam	Cat#ab236966; UniProt#Q4G0F5; https://www.abcam.com/vps26b-antibody-ab236966.html
Rabbit polyclonal anti-Tau Phospho-Thr231	Sigma-Aldrich	Cat#AB9668; UniProt#P10636; https://www.sigmaaldrich.com/DK/en/product/mm/ab9668
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fischer Scientific	Cat#A-21206; RRID: AB_2535792
Goat anti-Rabbit IgG/Biotin-Conjugated Secondary Antibody	Sigma-Aldrich	Cat#B8895; RRID: AB_258649
Rabbit anti-mouse IgG/HRP-Conjugated Secondary Antibody	Agilent	Cat#P0260; RRID: AB_2636929
Chemicals, peptides, and recombinant proteins
AmnioMax™ C-100 Complete Medium	Thermo Fischer Scientific	Cat#12558011
Human FGF-basic Recombinant Protein (bFGF)	Thermo Fischer Scientific	Cat#PHG0266
Critical commercial assays
V-Plex Aβ Peptide Panel 1 (6E10) Kit	MSD	Cat#K15200E
sAPPα/sAPPβ Kit	MSD	Cat#K15120E
Mouse Total Tau Kit	MSD	Cat#K151DSD
Simple Plex Human NF-L Cartridge	Protein Simple	Cat# SPCKB-PS-002448
Experimental models: Cell lines
Göttingen Minipigs Primary Fibroblasts	Ellegaard Göttingen Minipigs	https://minipigs.dk/
SORL1 Göttingen Minipigs Primary Fibroblasts	This study	N/A
Human Embryonic Kidney 293T Cell Line	ATCC	Cat#CRL-3216
Experimental models: Organisms/strains
Göttingen Minipigs	Ellegaard Göttingen Minipigs	https://minipigs.dk/
Oligonucleotides
Primers and probes	This paper	Table S2
Recombinant DNA
pNeDaKo-Neo plasmid	Bert Vogelstein Lab, The Johns Hopkins University Medical Institutions, Baltimore, MD, USA	https://doi.org/10.1093/nar/gnh009
pAAV-MCS vector	Agilent	Cat#240071
Human codon-optimized Cas9 plasmid	Mali et al., 2013, https://www.science.org/doi/10.1126/science.1232033	Addgene plasmid #41815
rAAV/SORL1 KO-Neo vector	This study	N/A
C-check vector	Zhou et al., 2016	https://doi.org/10.1007/s00018-015-2128-3
sgRNA1	This study	N/A
Software and algorithms
Zink Finger Targeter (ZiFiT)	Sander et al., 2010, https://doi.org/10.1093/nar/gkq319	http://bindr.gdcb.iastate.edu/ZiFiT/ (discontinued)
CRISPR RGEN Cas-OFFinder	Bae et al., 2014, https://doi.org/10.1093/bioinformatics/btu048	http://www.rgenome.net/cas-offinder/
ImageJ2	NIH	https://imagej.net/downloads
GraphPad Prism 6.0	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Other
BTX Microslide 0.5 mm Fusion Chamber, model 450	BTX	https://www.btxonline.com/
ELLA Automated Immunoassay System	Protein Simple	https://www.bio-techne.com/p/simple-plex/ella-automated-immunoassay-system_600-100