Autoradiograph of Southern blot probed with mitochondrial 12S rRNA gene fragment. Shown is a DNase I (40 μg/ml) reaction time course at RT (25°C). Lane 1, 15 min; lane 2, 22 min; lane 3, 28 min; lane 4, 35 min; lane 5, 40 min. Arrow, Drosophila mitochondrial genome fragment.