Extended Data Fig. 5. Temperature dependent ¹⁹F relaxation dispersion.

¹⁹F CPMG (left) and R_1ρ (right) relaxation dispersion of CtXrn2 1-875 ΔZnF N12C^BTFA at temperatures between 303 K and 313 K and ¹H Larmor frequencies of 500 MHz and 600 MHz. Fit parameters of the global fit and separate fits of CPMG and R_1ρ datasets are included in Table S6. Note that the R_1ρ data contains more datapoints at low frequencies. In the CPMG experiments the number of possible frequencies is determined by the relaxation delay and can only be a multiple of 1 / T_relax = 62.5 Hz (for the T_relax of 16 ms that was used). The R_1ρ experiment, on the other hand, can use arbitrary spin-lock frequencies and is thus able to sample the RD curve at low frequencies better. Note that very low spinlock frequencies cannot be used in R_1ρ experiment, as then the signal decay is no longer mono-exponential. The extracted fitting parameters, including errors are summarized in Supplementary Table 6. Data points are shown as mean ± s.d., as derived from 3 duplicate NMR measurements.