Table 2.
Data collection and refinement statistics
| SeMETTL4 | METTL4- SAM | METTL4- SAH | METTL4- SFG | METTL4-Am-SAH | |
|---|---|---|---|---|---|
| Space group | P21 | P21 | P21 | P212121 | I222 |
| a, b, c (Å) | 60.8,94.3, 95.7 | 61.9,93.7, 96.3 | 63.6,93.5, 96.6 | 52.2,84.6, 94.1 | 93.8,130.0, 141.3 |
| α, β, γ (°) | 90.0,101.7,90.0 | 90.0,104.3,90.0 | 90.0,104.7,90.0 | 90.0,90.0, 90.0 | 90.0,90.0, 90.0 |
| Wavelength(Å) | 0.97930 | 0.97916 | 0.97776 | 0.97853 | 0.97852 |
| Resolution (Å) | 30.0-2.50 | 30.0-2.30 | 30.0-2.45 | 30.0-2.35 | 30.0-3.0 |
| Last shell (Å) | (2.59-2.50)a | (2.38-2.30)a | (2.54-2.45)a | (2.43-2.35)a | (3.11-3.00)a |
| Completeness (%) | 98.2(93.7) | 98.1(91.1) | 97.6(88.6) | 98.8(91.5) | 82.4(54.7) |
| Redundancy | 4.8(3.3) | 3.4(3.1) | 5.3(4.0) | 11.8(8.6) | 5.8(2.4) |
| I/σ(I) | 29.9(7.2) | 25.6(2.0) | 17.5(2.4) | 24.2(3.3) | 7.8(1.9) |
| Rmerge (%) | 13.2(41.3) | 6.3(59.7) | 10.4(39.2) | 17.4(60.6) | 11.2(40.0) |
| No. reflections | 36127 | 46526 | 39010 | 17743 | 14468 |
| (3421) | (4278) | (3536) | (1596) | (938) a | |
| Refinement | |||||
| Resolution (Å) | 2.50 | 2.30 | 2.45 | 2.35 | 3.01 |
| Rwork (%) / Rfree (%) | 22.6/26.5 | 22.0/25.1 | 21.1/25.4 | 20.1/23.7 | 24.0/30.0 |
| No. of atoms | |||||
| Protein | 5326 | 5515 | 5639 | 2741 | 2877 |
| RNA/DNA | 0 | 0 | 0 | 0 | 48 |
| Ligand/ion | 0 | 54 | 96 | 45 | 26 |
| Water | 11 | 57 | 22 | 61 | 19 |
| B factors | 50.0 | 72.0 | 55.0 | 47.0 | 94.0 |
| Bond length (Å) | 0.007 | 0.006 | 0.005 | 0.003 | 0.019 |
| Bond angle (°) | 1.010 | 1.080 | 0.974 | 0.072 | 1.982 |
| Ramachandran plot (%) | |||||
| Most favored | 96.31 | 97.41 | 98.11 | 5.38 | 89.82 |
| Additional allowed | 3.69 | 2.59 | 1.89 | 4.62 | 10.15 |
| Outliers | 0 | 0 | 0 | 0 | 0 |
| PDB number | 7CVA | 7CV7 | 7CV9 | 7CV8 | 7CV6 |
Data are from one crystal for each structure.
aValues in parentheses are for highest-resolution shell.