FIG. 1.

Comparison of the amino acid sequences of S. pombe Alr1p (GenBank accession no. AL023590) with the bacterial alanine racemases (plus GenBank accession numbers) of B. stearothermophilus (M19142), E. coli Alr (AE00478), S. enterica serovar Typhimurium Alr (M12847), E. coli DadX (LO2948), S. enterica serovar Typhimurium DadB (KO2119), and E. gallinarum VanT (AF162694). The amino acid sequences were aligned with the MegAlign program of the DNASTAR package (5). Conserved residues are boxed. The open circle indicates the Lys residue that binds PLP and is proposed to abstract α-proton from d-alanine. The closed circle indicates the Tyr residue that is proposed to abstract α-hydrogen from l-alanine. Asterisks show the residues involved in binding with PLP.