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Western blot analysis of Alr1p. S. pombe SP1 cells exponentially growing on YPD medium (lane 1) or a minimum medium containing, as a sole nitrogen source, ammonium sulfate (lane 2), l-alanine (lane 3), or d-alanine (lane 4) were harvested. Cell extracts were subjected to SDS–13.0% PAGE, blotted onto a polyvinylidene difluoride membrane, and analyzed with polyclonal anti-Alr1p antibody. Each lane was loaded with 10 μg of protein.
