Fig. 4. Fer-1 and Lip-1 reduced the ferroptosis of MSC^SPIO.

MSCs were treated with Erastin (100 nmol/L) or RSL3 (10 nmol/L) or treated with Fer-1 (10 μmol/L) or Lip-1 (20 μmol/L) during SPIO labeling. Cells were harvested at 24 h. A Cell viability of MSCs under different treatment was measured by CCK-8 assay. B, D The intracellular ROS level was measured by B flow cytometry and D confocal microscope. C, E The intracellular level of lipid peroxide was measured by C flow cytometry and E confocal microscope. Scale bars, 200 μm. Data are shown as mean ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001.