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. 2022 Sep 26;13:5645. doi: 10.1038/s41467-022-33269-x

Table 2.

Group statistics for predicted responders and non-responders to anti-CD20-Abs at the 50th and 70th percentile thresholds, in the held-out test set

50th percentile thresholda 70th percentile thresholda
Responders Non-responders Effect size (95% CI)b p valuec Responders Non-responders Effect size (95% CI)b pvaluec
Trial contribution
OLYMPUS 55 54 35 74
ORATORIO 96 92 57 131
Demographics
Age (years) 45.20 (8.58) 47.84 (7.89) −2.64 (-4.53, −0.76) 0.006 44.59 (9.05) 47.36 (7.87) −2.77 (−4.93, −0.61) 0.013
Sex (% male) 47.02 50.68 0.86 (0.53, 1.40) 0.562 45.65 50.24 0.83 (0.49, 1.40) 0.530
Height (cm) 170.05 (10.56) 170.55 (8.80) −0.50 (−2.72, 1.71) 0.657 169.78 (10.29) 170.52 (9.47) −0.74 (−3.23, 1.75) 0.560
Weight (kg) 76.17 (18.93) 72.96 (13.77) 3.21 (−0.56, 6.98) 0.096 75.68 (20.07) 74.10 (14.87) 1.58 (−3.04, 6.20) 0.502
Disease duration (years) 6.07 (4.14) 8.72 (5.45) −2.65 (−3.76, −1.54) <0.001 5.79 (4.15) 8.09 (5.19) −2.30 (−3.41, −1.19) <0.001
Disability scores
EDSS 4.87 (1.18) 4.52 (1.23) 0.34 (0.07, 0.62) 0.015 5.07 (1.14) 4.53 (1.21) 0.54 (0.25, 0.83) <0.001
FSS-Bowel and Bladder 1.25 (0.93) 1.11 (0.80) 0.14 (−0.05, 0.34) 0.157 1.27 (0.98) 1.15 (0.82) 0.12 (−0.11, 0.35) 0.315
FSS-Brainstem 0.82 (0.93) 0.79 (0.87) 0.04 (−0.17, 0.24) 0.726 0.90 (0.95) 0.77 (0.88) 0.13 (−0.10, 0.36) 0.265
FSS-Cerebellar 2.38 (0.97) 1.78 (1.05) 0.60 (0.37, 0.83) <0.001 2.57 (0.81) 1.86 (1.08) 0.71 (0.48, 0.93) <0.001
FSS-Cerebral 1.07 (0.83) 1.05 (0.89) 0.02 (−0.18, 0.22) 0.848 1.13 (0.84) 1.04 (0.87) 0.09 (−0.12, 0.30) 0.404
FSS-Pyramidal 2.75 (0.69) 2.90 (0.58) −0.14 (−0.29, 0.00) 0.052 2.77 (0.76) 2.85 (0.58) −0.08 (−0.26, 0.10) 0.382
FSS-Sensory 1.55 (1.06) 1.64 (1.02) −0.08 (−0.32, 0.15) 0.488 1.56 (1.00) 1.61 (1.06) −0.05 (−0.30, 0.20) 0.703
FSS-Visual 1.04 (1.04) 0.43 (0.62) 0.62 (0.42, 0.81) <0.001 1.28 (1.07) 0.50 (0.71) 0.78 (0.54, 1.02) <0.001
Mean T25FW (s) 13.55 (17.61) 10.75 (11.08) 2.80 (−0.55, 6.15) 0.103 15.95 (21.79) 10.48 (9.82) 5.47 (0.77, 10.17) 0.024
Mean 9HPT dominant (s) 32.62 (26.89) 26.70 (10.24) 5.92 (1.29, 10.55) 0.013 36.01 (33.25) 26.88 (9.89) 9.13 (2.12, 16.15) 0.012
Mean 9HPT non-dominant (s) 37.33 (31.11) 26.97 (9.32) 10.36 (5.14, 15.58) <0.001 42.39 (38.33) 27.68 (9.33) 14.71 (6.68, 22.75) <0.001
MRI metrics
Gad count 1.62 (3.14) 0.16 (0.48) 1.46 (0.95, 1.97) <0.001 1.90 (3.64) 0.46 (1.27) 1.44 (0.67, 2.22) <0.001
T2 lesion volume (mL) 13.09 (12.85) 7.72 (10.17) 5.37 (2.73, 8.01) <0.001 14.31 (14.22) 8.72 (10.27) 5.59 (2.33, 8.85) <0.001
Normalized brain volume (L) 1.37 (0.16) 1.38 (0.16) −0.02 (−0.05, 0.02) 0.367 1.35 (0.16) 1.38 (0.16) −0.03 (−0.07, 0.01) 0.107

Values in brackets are standard deviations, unless otherwise specified.

EDSS Expanded Disability Status Scale, FSS Functional Systems Score, T25FW timed 25-foot walk, 9HPT 9-hole peg test, Gad Gadolinium-enhancing lesion.

a Percentile threshold for defining responders. The 50th percentile defines responders as the top 50% who are predicted to be most responsive, while the 70th percentile defines them as the top 30%. The non-responders are those who fall below the percentile threshold.

b Effect size is the average difference between responders and non-responders for all covariates except for "sex” which is an odd’s ratio (OR).

c p values for continuous and ordinal variables are calculated using a two-sided Welch’s t test due to unequal variances/sample sizes. p value for the categorical variable "sex” is calculated using a two-sided Fisher’s exact test due to unequal and relatively small sample sizes. Exact p-values for the 50th percentile threshold: disease duration, p = 4.39 × 10−6; FSS-Cerebellar, p = 6.42 × 10−7; FSS-Visual, p = 2.18 × 10−9; Mean 9HPT non-dominant, p = 1.36 × 10−4; Gad count, p = 8.72 × 10−8; T2 lesion volume, p = 8.57 × 10−5. Exact p-values for the 70th percentile threshold: disease duration, p = 7.04 × 10−5; EDSS, p = 3.03 × 10−4; FSS-Cerebellar, p = 2.61 × 10−9; FSS-Visual, p = 3.38 × 10−9; Mean 9HPT non-dominant, p = 4.82 × 10−4; Gad count, p = 3.69 × 10−4; T2 lesion volume, p = 9.59 × 10−4.