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. 2022 Sep 26;13:5657. doi: 10.1038/s41467-022-33410-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a KEGG analysis of down-regulated genes in GDYO-treated OCI-AML3 cells. b Fragments Per Kilobase per Million (FPKMs) of genes related in actin cytoskeleton in control and GDYO groups. n = 3 biologically independent experiments. c Silver staining of SDS-PAGE gel loaded with GDYO-treated cell lysates. d Antibody-guided immunoblot for binding ability between β-actin and GDYO nanosheets. e Simulated binding site of GDYO (cyan), cytochalasin D (green) and latrunculin A (blue) onto the actin monomer. f-g G-actin polymerization (f) and F-actin depolymerization (g) curves treated with GDYO, cytochalasin D and latrunculin A. The numbers in brackets indicated the percentages of polymerization or depolymerization (%). h Live cell imaging of actin with DIC component in GDYO-treated OCI-AML3 cells. Arrows indicated the co-localizations of GDYO nanosheets and actin. Scale bar, 5 μm. i Western blot analysis of F-actin and G-actin after GO or GDYO treatment. j Representative SEM images of OCI-AML3 treated by GO or GDYO for 6, 24 and 48 h. Scale bar, 5 μm. k Representative F-actin immunofluorescence confocal images of OCI-AML3 treated by saline, Jas, GDYO, Jas and GDYO for 48 h. Scale bar, 10 μm. l-m Fold change of apoptosis (l) and CFU (m) for GDYO v.s. control is rescued by Jas. n = 3 biologically independent experiments. The data were shown as the mean ± SD. Statistical significance was tested with a two-tailed, unpaired Student’s t test. Source data are provided as a Source Data file.