FIGURE 11.

Silencing GhGAD6 via VIGS increased sensitivity to Cd²⁺ stress. (A) The phenotype of cotton after GhGAD6 gene silencing under Cd²⁺ stress. pYL156:PDS as a positive control, pYL156 was an empty vector as control, and pYL156:GhGAD6 was GhGAD6 silenced lines.(B) The relative expression level of GhGAD6 under water and Cd²⁺ stress. (C) DAB staining. (D) ChlorophylⅡ content of empty control and VIGS plants under Cd²⁺ stress. (E) SOD activity of empty control and VIGS plants under Cd²⁺ stress. (F) Detection of gene expression of GABA shunt in GhGAD6 silenced lines. The black box represents the darkening of the leaf veins, the black arrows indicate the location of H₂O₂ generation.*0.01 < p < 0.05, **p < 0.01.