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. 2022 Sep 23;22:1–17. doi: 10.1016/j.bioactmat.2022.08.025

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Proliferation and migration evaluation of the BMSCs with different hydrogels. (A) CCK-8 assay evaluating the cytotoxic effects after 1–7 days co-incubation with different hydrogels. (B) Hemolysis test for the RSF-based hydrogels. PC, a positive control (distilled water); NC, a negative control (normal saline). (C) Living/dead staining on different hydrogels with varying ES potentials. (D) Immunofluorescence staining results of Ki67 proteins. (E) A custom-designed device to detect cell migration using hydrogel electrodes. (F) Wound scratch assay in different hydrogel samples with or without ES potential. **P < 0.01, *P < 0.05, n = 3.