FIGURE 8.

Confirmation of the inhibitory function of PES on PfHop-PfHsp70-1 association. Representative sensorgrams generated from data obtained in absence of nucleotide (A), presence of ADP (B), ATP (C), and PES (D) and EGCG (E) are shown. The relative affinities of the association of PfHop with PfHsp70-1 in the absence (NN) of both nucleotides and inhibitors compared to interaction in the presence of nucleotides and inhibitors are shown in (F). Standard errors represent three independent assays conducted in triplicates using independent protein batches. A student t-test was used for statistical validation and statistically significant differences relative to data obtained in the absence of nucleotide state (NN) are indicated by asterisks above the bar graphs (p < 0.001*).