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. 2022 Sep 13;9:947203. doi: 10.3389/fmolb.2022.947203

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Copyright © 2022 Muthelo, Mulaudzi, Netshishivhe, Dongola, Kok, Makumire, de Villiers, Burger, Zininga and Shonhai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The normalized dose-response curve for the in vitro susceptibility of P. falciparum to PES. The in vitro antiplasmodial activity of PES was investigated using a SYBR™ Gold DNA stain to measure the proliferation of the parasites as compared to the controls. Fluorescence was measured using a TECAN Spark^® multimode microplate reader and the data analysis was done using SigmaPlot, version 12 (Systat Software Inc., Chicago, IL, United States). IC₅₀ determinations were performed as technical triplicates for three biological repeats with standard error of the mean shown.