TABLE 5.
Fold induction of specific transcripts attributed to sdiA amplification as determined by probing of microarrays and amplification of cDNA samples
| Gene | Fold changea
|
|
|---|---|---|
| Amplification | Probing | |
| sdiA | 12.7 | 30.0 |
| b1707 | 8.9 | 30.0 |
| b1606 | 4.8 | 8.0 |
| uvrY | 4.9 | 11.9 |
| uvrC | 4.1 | 9.3 |
| gusR | 3.2 | 8.0 |
| nei | 3.2 | 8.6 |
| ybgL | 2.7 | 5.2 |
| ybgK | 2.4 | 4.7 |
| ybgJ | 2.4 | 6.4 |
| ybgI | 2.1 | 3.8 |
| acrA | 2.3 | 6.8 |
| acrB | 2.0 | ND |
| dniR | 2.0 | 5.8 |
| feoA | 2.0 | 5.8 |
| mioC | 1.9 | 7.0 |
| b0517 | 1.4 | 11.2 |
| acrR | 0.8 | 4.5 |
| icdA | 1.1 | <1.5 |
| mdh | 1.1 | <1.5 |
a
Amplification results were obtained by reverse transcription of bulk RNA followed by PCR amplification. Probing results were obtained with fluorescently labeled cDNA hybridized to DNA microarrays. ND, not determined.