Figure 6.

SARS-CoV-2 recent infection alters the phenotype of spike-specific B cells

(A) Representative expression of CD21 and CD27 among RBD⁺ B cells at baseline and day 60 post-boost. Numbers represent percentages.

(B) Frequencies of CD21^lo and CD21⁺CD27^lo RBD⁺ B cells at baseline and day 60 post-boost.

(C) Baseline uninfected anti-BCR-induced phosphorylation of Syk among CD21⁺CD27^lo and CD21⁺CD27⁺ RBD⁺ B cells.

Populations shown in (A) and frequencies measured in (B) were from the CD19⁺ gate shown in Figure S3A and those in (C) further gated on CD21⁺ B cells. Horizontal lines represent medians and coloring scheme matches groups described in Figure 1A. Gray circles refer to subject 00065 R in Table S1 and Figure S5A. Statistical analyses by Wilcoxon signed rank (B for baseline versus day 60 and C) or Mann-Whitney test (B for uninfected versus prior-infected). ^∗p ≤ 0.05, ^∗∗p ≤ 0.01.