KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| SerpinB3 | Invitrogen | PA5-30164; RRID:AB_2547638 |
| JAM-A | B&D Biosciences | 612120; RRID:AB_399491 |
| cathepsin L | ThermoFisher | BMS1032; RRID:AB_10596643 |
| c-Myc | Cell Signaling Technology | 5605; RRID:AB_1903938 |
| β-Actin | Bio-Rad | 12004163; RRID:AB_2861334 |
| Rabbit StarBright 700 | Bio-Rad | 12004161; RRID:AB_2721073 |
| JAM-A | Santa Cruz | sc-53623; RRID:AB_784134 |
| SOX2 | R&D | MAB2018; RRID:AB_358009 |
| AKT | Cell Signaling | 9272; RRID:AB_329827 |
| pAKT(Ser473) | Cell Signaling | 4075S; RRID:AB_916029 |
| Donkey-anti mouse Alexa Fluor 555 | ThermoFisher | A-31570; RRID:AB_2536180 |
| Donkey-anti rabbit Alexa Fluor 488 | ThermoFisher | A-21206; RRID:AB_2535792 |
| JAM-A AF647 | Santa Cruz | sc-53623 AF647; RRID:AB_784134 |
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| Biological samples | ||
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| GBM Fresh Tumor Samples | Cleveland Clinic Foundation and Northwestern University | This manuscript |
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| Chemicals, peptides, and recombinant proteins | ||
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| N-terminal His-tagged full-length JAM-A | Sinobiologicals | HG10198-NH |
| Hoechst 33342 | Invitrogen | H3570 |
| Vectashield | Vector Labs | H-1000-10 |
| acridine orange | Sigma | A6014 |
| pH6 retrieval buffer (Reveal) | Biocare | V1000 |
| serum-free casein background block (Background Sniper) | Biocare | BS966 |
| MACH 4 Universal HRP Polymer | Biocare | M4U534 |
| Geltrex | Life Technologies | A1413301 |
| puromycin | ThermoFisher | 54-022-2100 |
| L-leucyl-L-leucine methyl ester | Cayman | #16008 |
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| Critical commercial assays | ||
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| Betazoid DAB Chromogen Kit | Biocare | BDB2004 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7570 |
| Caspase-Glo 3/7 | Promega | G8090 |
| RNeasy mini kit | Qiagen | 74004 |
| nCounter® PanCancer Pathways Panel | Nanostring | N/A |
| TGF-β1 DuoSet ELISA | R&D systems | DY240 |
| FITC-labeled annexin V and propidium iodide | BioLegend | 640914 |
| Caspase 3/7 | Sartorius | 4704 |
| qScript synthesis reagent | Quanta Biosciences | 95048 |
| SYBR-Green Mastermix | SA Biosciences | 4385610 |
| E64D | Enzo | BML-PI107-0001 |
| Z-FY-CHO | Med Chem Express | HY-128140 |
| Cycloheximide | Alfa Aesar | J66004 |
| LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit | Invitrogen | L23105 |
| MojoSort Buffer (5X) | Biolegend | 480017 |
| eBioscience™ Fixation/Permeabilization | Invitrogen | 00-5523 |
| MycoAlert detection kit | Lonza | LT07-118 |
| CD133 selection beads | Miltenyi | 130-097-049 |
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| Deposited data | ||
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| Unaltered Blots | Mendeley data | https://doi.org/10.17632/6dwf49mg73.1 |
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| Experimental models: Cell lines | ||
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| 293T | DuBridge et al., 1987 | Obtained internally, available https://www.atcc.org/products/crl-3216 |
| T387 | Bao et al., 2008 | N/A |
| T4121 | Bao et al., 2008 | N/A |
| DI318 | This manuscript | N/A |
| Human astrocytes | ScienCell Research Laboratories | #1800 |
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| Experimental models: Organisms/strains | ||
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| NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) | Jackson Labs | 005557 |
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| Oligonucleotides | ||
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| SERPINB3 F- CGCGGTCTCGTGCTATCTGG, R- AGAAGAGGATGCTGTTGGTC |
This manuscript | N/A |
| OCT4 F- TGAGTCAGTGAACAGGGAATG R- AATCTCCCCTTTCCATTCGG |
This manuscript | N/A |
| NANOG F- GAAATACCTCAGCCTCCAGC R- GCGTCACACCATTGCTATTC |
This manuscript | N/A |
| OLIG 2 F- AGCTCCTCAAATCGCATCC R-AAAAGGTCATCGGGCTCTG |
This manuscript | N/A |
| c-MYC F- TTCGGGTAGTGGAAAACCAG R- AGTAGAAATACGGCTGCACC |
This manuscript | N/A |
| TGF-β1 F- AAGTGGACATCAAGGGTTC R- GTCCTTGCGGAAGTCAATGT |
This manuscript | N/A |
| GAPDH F-ACATCGCTCAGACACCATG R- TGTAGTTGAGGTCAATGAAGGG |
This manuscript | N/A |
| NOTCH2 F- GTGCCTATGTCCATCTGGATGG R- AGACACCTGAGTGCTGGCACAA |
This manuscript | N/A |
| JAGGED2 F- GCTGCTACGACCTGGTCAATGA R- AGGTGTAGGCATCGCACTGGAA |
This manuscript | N/A |
| Primers also listed in Table S2 | This manuscript | N/A |
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| Recombinant DNA | ||
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| shRNA Control Plasmid (SHC002) | Sigma | SHC002 |
| SerpinB3 KD1 | Sigma | TRCN0000373440 |
| SerpinB3 KD2 | Sigma | TRCN0000373501 |
| SerpinB3 KD3 | Sigma | TRCN0000052398 |
| SerpinB3 KD4 | Sigma | TRCN0000373500 |
| JAM-A KD1 | Sigma | TRCN0000061650 |
| JAM-A KD2 | Sigma | TRCN0000061649 |
| psPAX2 | Addgene | #12260; RRID:Addgene_12260 |
| pMD2G | Addgene | #12259; RRID:Addgene_12259 |
| pLPC-N FLAG vector | Addgene | #12521; RRID:Addgene_12521 |
| Fugene HD | Promega | E2311 |
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| Software and algorithms | ||
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| LASX software | Leica | N/A |
| IncuCyte SX5 Live-Cell Analysis Instrument | Sartorius | https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-instruments/sx5-live-cell-analysis-instrument |
| nSolver version 4.0 | Nanostring | N/A |
| ELDA | Hu and Smyth, 2009 | http://bioinf.wehi.edu.au/software/elda/index.html |
| Applied Biosystems QuantStudio 3 | Thermo Fisher | N/A |
| Fiji | Schindelin et al., 2012 | https://imagej.net/downloads |
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| Other | ||
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| Depmap | Tsherniak et al., 2017 | https://depmap.org/R2-D2/ |