Table 2.
Primer sequences used for RT-qPCR.
| Protein | Gene | Sense | Gene Sequence (5’ → 3’) | Tm (°C) | NCBI GeneID |
|---|---|---|---|---|---|
| Angiotensin Converting Enzyme 2 | ACE2 | Forward Reverse |
ACAGTCCACACTTGCCCAAAT TGAGAGCACTGAAGACCCATT |
60 | NM_001371415.1 |
| Transmembrane Serine Protease 2 | TMPRSS2 | Forward Reverse |
GAT TAG CCG TCT GCC CTC ATT TGT CAG GAG TGT ACG GGA ATG TGA TGG |
58 | NM_001135099.1 |
| BSG basigin | CD147 | Forward Reverse |
CTA CAC ATT GAG AAC CTG AAC TTC TCG TAG ATG AAG ATG ATG |
60 | NM_001728.4 |
| Interleukin 6 | IL-6 | Forward Reverse |
GTA CAT CCT CGA CGG CAT C CCA GGC AAG TCT CCT CAT TG |
60 | NM_000600.3 |
| C-X-C motif chemokine ligand 8 | IL8/CXCL8 | Forward Reverse |
AGG TGC AGT AGT TTT GCC AAG GA TTT CTG TGT TGG CGC AGT GT |
60 | NM_000584 |
| Tumor Necrosis Factor-α | TNF | Forward Reverse |
GGC CCA GGC AGT CAG ATC AT GGG GCT CTT GAT GGC AGA GA |
65 | NM_000594.3 |
| C-C motif chemokine ligand 2 | MCP1/CCL2 | Forward Reverse |
ATC AAT GCC CCA GTC ACC AGT CTT CGG AGT TTG GG |
60 | NM_002982.3 |
| CCL5 C-C motif chemokine ligand 5 | RANTES/CCL5 | Forward Reverse |
TAC CAT GAA GGT CTC CGC GAC AAA GAC GAC TGC TGG |
60 | NM_002985.3 |
| C-X-C motif chemokine ligand 10 | IP10/CXCL10 | Forward Reverse |
AGG AAC CTC CAG TCT CAG CA CAA AAT TGG CTT GCA GGA AT |
60 | NM_001565.4 |
| Ribosomal protein S18 | RPS18 | Forward Reverse |
ATC CCT GAA AAG TTC CAG CA CCC TGT TGG TGA GGT CAA TG |
60 | NM_022551.2 |
| Actin Beta | ACTB | Forward Reverse |
CTA CAA TGA GCT CCG TGT GG AAG GAA GGC TGG AAG AGT GC |
60 | NM_001101.3 |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | Forward Reverse |
GAT CAT CAG CAA TGC CTC CT GT GGT CAT GAG TCC TTC CA |
60 | NM_002046.5 |
| SARS-CoV-2 Nucleocapsid Protein | 2019-nCoV_N2 | Forward Reverse Probe |
TTA CAA ACA TTG GCC GCA AA GCG CGA CAT TCC GAA GAA FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 |
43740575 |