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Optimization of expression conditions: (A) investigation of added silica gel in per 50 μL system, (B) investigation of expression time: observe the amount of inserted and not-inserted PDGFRβ at 1 or 2 h expression time, (C) investigation of insertion time given to PDGFRβ, (D) investigation of maximum proportion of DOPE, (E) quantification analysis of bands in (C), (F) quantification analysis of bands in (D).
