. 2022 Mar 31;12(9):3548–3566. doi: 10.1016/j.apsb.2022.03.019

Table 1.

The discovery strategies of molecular glues and the specific techniques exploited within these studies.

Target protein	Molecular glues	Discovery strategy	Techniques	Refs.
PD-L1	BMS-202	Serendipity	NMR, DSF, crystal	32
REV1	JH-RE-06	Serendipity	Crystal, SDS-PAGE	40
MDMX	RO-2443, RO-5986	Serendipity	NMR, SEC-SLS, crystal, TR-FRET	46
BCL6	BI-3802	Serendipity	IF, Cryo-EM, WB, TR-FRET	53
BCL6	CCT369260	Serendipity	Nano-BRET, IF, MSD	54
MERS-CoV	P3	Virtual screening	SAXS, IF, crystal, thermostability	61
DDB1^CRBN–substrate	Thalidomide, lenalidomide, pomalidomide	Serendipity	Proteomic, LC‒MS/MS, crystal, TR-FRET	62,63
	CC-122, CC-220	Rational design	Crystal, WB, TR-FRET	64,65
	CC-885	Rational design	IP, crystal, pull-down, IF, LC‒MS/MS, SPPIER	67
	CC-90009	Rational design	WB, proteomic, crystal	69
	SJ6986	Rational design	WB, MD simulation	68
DDB1^DCAF15–RBM39	Indisulam, E7820	Serendipity	IP, LC‒MS/MS, CRISPR/Cas9, pull-down, Cryo-EM, TR-FRET	77,78
DDB1–CDK12/cyclinK	dCeMM2, dCeMM3, dCeMM4	Chemical screening	WB, CRISPR/Cas9, pull-down	83
	(R)-CR8	Data mining	Co-IP, ITC, crystal, TR-FRET, nano-BRET	85
	HQ461	HTS	CRISPR/Cas9, Co-IP, WB, pull-down, CXMS, LC‒MS/MS, DSF, thermostability	84
SCF^β-TrCP–β-catenin	NRX-252114, NRX-252262, NRX-103094	HTS, rational design	FP, crystal, TR-FRET, pull-down	89
LC3-mHTT	10O5, 8F20, AN1, AN2	HTS	SMM, OI-RD, HTRF, MST, pull-down, IF	93
GLP-1–GLP-1R	LSN3160440	HTS, rational design	Crystal, MD simulation, IF	101