Skip to main content
. Author manuscript; available in PMC: 2023 Apr 8.
Published in final edited form as: Science. 2022 Apr 8;376(6589):eabl5282. doi: 10.1126/science.abl5282

Figure 1. The design of catch bond fishing libraries and selection strategy.

Figure 1.

(A) TCR55-transduced SKW3 T cells were stimulated by KG-1 cells pulsed with titrated HIV or Pep20 peptides for 14 hours. Anti-CD69 staining was performed on the SKW3 T cells and analyzed by flow cytometry.

(B) TCR55-transduced SKW3 T cells were stimulated by KG-1 cells pulsed with titrated HIV or Pep20 peptides for 15 min. Anti-phospho-ERK staining was performed on the SKW3 T cells and analyzed by flow cytometry.

(C) The design of TCR55 libraries. Each library has 3 or 4 residues selected to be randomized. The side chains of the residues selected for mutation on TCR55 are shown as sticks in the figures.

(D) Workflow of catch bond engineering of TCR.

(A-B) Data are representative of 3 independent experiments. Data are shown as mean ± SD of technical triplicates.